Stable Isotope Dilution Assays as Tools for Evaluating Vitamin Contents and Bioactivity
نویسنده
چکیده
For examining vitamins, exact quantitations in natural matrices are essential. Up to date, one of the most accurate quantitation methods is the stable isotope dilution assay (SIDA), which is based on the use of isotopically labelled analogues of the analytes as internal standards. The merits of SIDA include the ideal compensation for losses, superior specificity and enhanced sensitivity due to the “carrier effect” [1]. Therefore, SIDAs are superior to other assays for vitamins that occur in low concentrations and are labile during analysis. One of these groups of vitamins is the folates.
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